Detailed information of TA system TA072048

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4454855..4455562
• Replicon: chromosome
• Accession: NZ_CP019259
• Organism: Escherichia coli strain 13C1065T

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A377AQ31
• Locus tag: BWI86_RS23050
• Protein ID: WP_032161355.1
• Coordinates: 4454855..4455196 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BWI86_RS23055
• Protein ID: WP_032161354.1
• Coordinates: 4455227..4455562 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI86_RS23010	4450826..4451476	-	651	WP_001037977.1	HNH endonuclease	-
BWI86_RS23015	4451613..4451756	+	144	Protein_4170	HNH endonuclease	-
BWI86_RS23030	4452398..4453186	-	789	WP_032161357.1	helix-turn-helix domain-containing protein	-
BWI86_RS23035	4453303..4453578	-	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BWI86_RS23040	4453582..4453830	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
BWI86_RS23045	4453907..4454740	-	834	WP_032250394.1	DUF4942 domain-containing protein	-
BWI86_RS23050	4454855..4455196	-	342	WP_032161355.1	TA system toxin CbtA family protein	Toxin
BWI86_RS23055	4455227..4455562	-	336	WP_032161354.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BWI86_RS23060	4455562..4456035	-	474	WP_032161353.1	DNA repair protein RadC	-
BWI86_RS23065	4456065..4456883	-	819	WP_001683514.1	DUF945 domain-containing protein	-
BWI86_RS23070	4457119..4458072	-	954	WP_032161351.1	hypothetical protein	-
BWI86_RS23075	4458665..4459279	+	615	WP_148116414.1	inovirus Gp2 family protein	-
BWI86_RS23080	4459397..4459615	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE	4446955..4463857	16902

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13048.01 Da
• Isoelectric Point: 9.8953

>T72048 WP_032161355.1 NZ_CP019259:c4455196-4454855 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLTRLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRSHNTTAR

Nucleotide

• Length: 342 bp

>T72048 NZ_CP019259:c4455196-4454855 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGAC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCAGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGTCGCAG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12530.56 Da
• Isoelectric Point: 8.1281

>AT72048 WP_032161354.1 NZ_CP019259:c4455562-4455227 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSACQKR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT72048 NZ_CP019259:c4455562-4455227 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCGCCTGCCAAAAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A377AQ31