Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1332752..1333406 Replicon chromosome
Accession NZ_CP019259
Organism Escherichia coli strain 13C1065T

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BWI86_RS06765 Protein ID WP_000244777.1
Coordinates 1332999..1333406 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWI86_RS06760 Protein ID WP_000354046.1
Coordinates 1332752..1333018 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI86_RS06735 1327921..1328664 + 744 WP_000951964.1 SDR family oxidoreductase -
BWI86_RS06740 1328721..1330154 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BWI86_RS06745 1330199..1330510 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BWI86_RS06750 1330674..1331333 + 660 WP_000250274.1 hemolysin III family protein -
BWI86_RS06755 1331529..1332509 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BWI86_RS06760 1332752..1333018 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWI86_RS06765 1332999..1333406 + 408 WP_000244777.1 toxin CptA Toxin
BWI86_RS06770 1333446..1333967 - 522 WP_001055874.1 flavodoxin FldB -
BWI86_RS06775 1334079..1334975 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BWI86_RS06780 1335000..1335710 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWI86_RS06785 1335716..1337449 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T72031 WP_000244777.1 NZ_CP019259:1332999-1333406 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T72031 NZ_CP019259:1332999-1333406 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT72031 WP_000354046.1 NZ_CP019259:1332752-1333018 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT72031 NZ_CP019259:1332752-1333018 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References