Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4168878..4169516 Replicon chromosome
Accession NZ_CP019256
Organism Escherichia coli strain 13TMH22

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BWI85_RS21510 Protein ID WP_000813794.1
Coordinates 4169340..4169516 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BWI85_RS21505 Protein ID WP_001270286.1
Coordinates 4168878..4169294 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI85_RS21485 4164030..4164971 - 942 WP_001251315.1 ABC transporter permease -
BWI85_RS21490 4164972..4165985 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
BWI85_RS21495 4166003..4167148 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
BWI85_RS21500 4167393..4168799 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
BWI85_RS21505 4168878..4169294 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BWI85_RS21510 4169340..4169516 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BWI85_RS21515 4169738..4169968 + 231 WP_000494239.1 YncJ family protein -
BWI85_RS21520 4170060..4172021 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BWI85_RS21525 4172094..4172630 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
BWI85_RS21530 4172683..4173897 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T72027 WP_000813794.1 NZ_CP019256:c4169516-4169340 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T72027 NZ_CP019256:c4169516-4169340 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT72027 WP_001270286.1 NZ_CP019256:c4169294-4168878 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT72027 NZ_CP019256:c4169294-4168878 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGTGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References