Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1934677..1935308 Replicon chromosome
Accession NZ_CP019256
Organism Escherichia coli strain 13TMH22

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag BWI85_RS10000 Protein ID WP_001259385.1
Coordinates 1934677..1934952 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag BWI85_RS10005 Protein ID WP_000593554.1
Coordinates 1934949..1935308 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI85_RS09985 1929681..1930748 + 1068 WP_000361467.1 HlyD family secretion protein -
BWI85_RS09990 1930745..1933480 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
BWI85_RS09995 1933480..1934604 + 1125 WP_001314210.1 ABC transporter permease -
BWI85_RS10000 1934677..1934952 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
BWI85_RS10005 1934949..1935308 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BWI85_RS10010 1935358..1936218 - 861 WP_001131782.1 class II aldolase -
BWI85_RS10015 1936250..1936519 - 270 WP_000933060.1 HPr family phosphocarrier protein -
BWI85_RS10020 1936509..1938017 - 1509 WP_001298718.1 carbohydrate kinase -
BWI85_RS10025 1938010..1939368 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
BWI85_RS10030 1939445..1939726 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
BWI85_RS10035 1939723..1940196 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1927060..1941567 14507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T72012 WP_001259385.1 NZ_CP019256:1934677-1934952 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T72012 NZ_CP019256:1934677-1934952 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT72012 WP_000593554.1 NZ_CP019256:1934949-1935308 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT72012 NZ_CP019256:1934949-1935308 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References