Detailed information of TA system TA072007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 472554..473391
• Replicon: chromosome
• Accession: NZ_CP019256
• Organism: Escherichia coli strain 13TMH22

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: BWI85_RS02565
• Protein ID: WP_096262872.1
• Coordinates: 472849..473391 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BWI85_RS02560
• Protein ID: WP_001297137.1
• Coordinates: 472554..472865 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI85_RS02535	467574..468521	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
BWI85_RS02540	468543..470534	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BWI85_RS02545	470524..471138	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BWI85_RS02550	471138..471467	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BWI85_RS02555	471479..472369	+	891	WP_000971336.1	heme o synthase	-
BWI85_RS02560	472554..472865	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BWI85_RS02565	472849..473391	+	543	WP_096262872.1	GNAT family N-acetyltransferase	Toxin
BWI85_RS02570	473447..474382	-	936	WP_001297127.1	sel1 repeat family protein	-
BWI85_RS02575	474790..476154	+	1365	WP_001000978.1	MFS transporter	-
BWI85_RS02580	476282..476773	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19804.03 Da
• Isoelectric Point: 8.6178

>T72007 WP_096262872.1 NZ_CP019256:472849-473391 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSNITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T72007 NZ_CP019256:472849-473391 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTAATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT72007 WP_001297137.1 NZ_CP019256:472554-472865 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT72007 NZ_CP019256:472554-472865 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3