Detailed information of TA system TA072004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 65556..66148
• Replicon: plasmid p13KWH46-4
• Accession: NZ_CP019254
• Organism: Escherichia coli strain 13KWH46

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: BWI84_RS28360
• Protein ID: WP_000513532.1
• Coordinates: 65900..66148 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: BWI84_RS28355
• Protein ID: WP_000681609.1
• Coordinates: 65556..65903 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI84_RS28315	60848..61189	-	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-
BWI84_RS28320	61557..61958	+	402	WP_021575837.1	hypothetical protein	-
BWI84_RS28325	61976..62728	+	753	WP_000797966.1	conjugal transfer protein TraL	-
BWI84_RS28330	62730..63437	+	708	WP_000108794.1	hypothetical protein	-
BWI84_RS28335	63457..63897	+	441	WP_023223352.1	hypothetical protein	-
BWI84_RS28340	63899..64225	+	327	WP_000206697.1	hypothetical protein	-
BWI84_RS28345	64485..64784	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
BWI84_RS28350	65286..65468	+	183	WP_001428470.1	hypothetical protein	-
BWI84_RS28355	65556..65903	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BWI84_RS28360	65900..66148	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
BWI84_RS28365	66637..66930	-	294	WP_000673428.1	hypothetical protein	-
BWI84_RS28370	67046..67228	-	183	WP_071599349.1	hypothetical protein	-
BWI84_RS28375	67501..67743	-	243	WP_000151456.1	hypothetical protein	-
BWI84_RS28380	67740..67994	-	255	WP_001230709.1	hypothetical protein	-
BWI84_RS28385	67991..68533	-	543	WP_001083904.1	hypothetical protein	-
BWI84_RS28390	68637..69152	-	516	WP_001025393.1	J domain-containing protein	-
BWI84_RS28400	69809..70081	+	273	WP_000019163.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..76784	76784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T72004 WP_000513532.1 NZ_CP019254:c66148-65900 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T72004 NZ_CP019254:c66148-65900 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT72004 WP_000681609.1 NZ_CP019254:c65903-65556 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT72004 NZ_CP019254:c65903-65556 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49