Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 3155..3648 Replicon plasmid p13KWH46-4
Accession NZ_CP019254
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag BWI84_RS27960 Protein ID WP_001230709.1
Coordinates 3394..3648 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag BWI84_RS27955 Protein ID WP_000151456.1
Coordinates 3155..3397 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS27925 138..437 - 300 WP_148117003.1 type II toxin-antitoxin system HicB family antitoxin -
BWI84_RS27930 939..1121 + 183 WP_001428470.1 hypothetical protein -
BWI84_RS27935 1209..1556 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
BWI84_RS27940 1553..1801 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
BWI84_RS27945 2290..2583 - 294 WP_000673428.1 hypothetical protein -
BWI84_RS27955 3155..3397 - 243 WP_000151456.1 hypothetical protein Antitoxin
BWI84_RS27960 3394..3648 - 255 WP_001230709.1 hypothetical protein Toxin
BWI84_RS27965 3645..4187 - 543 WP_001083904.1 hypothetical protein -
BWI84_RS27970 4291..4806 - 516 WP_001025393.1 J domain-containing protein -
BWI84_RS27980 5462..5734 + 273 WP_000019163.1 hypothetical protein -
BWI84_RS27985 5715..6923 + 1209 WP_000198533.1 IS91 family transposase -
BWI84_RS27995 7214..7402 + 189 WP_000957857.1 hypothetical protein -
BWI84_RS28000 7412..8611 + 1200 WP_000948429.1 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..76784 76784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-125)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-37)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T72003 WP_001230709.1 NZ_CP019254:c3648-3394 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T72003 NZ_CP019254:c3648-3394 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT72003 WP_000151456.1 NZ_CP019254:c3397-3155 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT72003 NZ_CP019254:c3397-3155 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References