Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1209..1801 Replicon plasmid p13KWH46-4
Accession NZ_CP019254
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag BWI84_RS27940 Protein ID WP_000513532.1
Coordinates 1553..1801 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag BWI84_RS27935 Protein ID WP_000681609.1
Coordinates 1209..1556 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS27925 138..437 - 300 WP_148117003.1 type II toxin-antitoxin system HicB family antitoxin -
BWI84_RS27930 939..1121 + 183 WP_001428470.1 hypothetical protein -
BWI84_RS27935 1209..1556 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BWI84_RS27940 1553..1801 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
BWI84_RS27945 2290..2583 - 294 WP_000673428.1 hypothetical protein -
BWI84_RS27955 3155..3397 - 243 WP_000151456.1 hypothetical protein -
BWI84_RS27960 3394..3648 - 255 WP_001230709.1 hypothetical protein -
BWI84_RS27965 3645..4187 - 543 WP_001083904.1 hypothetical protein -
BWI84_RS27970 4291..4806 - 516 WP_001025393.1 J domain-containing protein -
BWI84_RS27980 5462..5734 + 273 WP_000019163.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..76784 76784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T72002 WP_000513532.1 NZ_CP019254:c1801-1553 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T72002 NZ_CP019254:c1801-1553 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT72002 WP_000681609.1 NZ_CP019254:c1556-1209 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT72002 NZ_CP019254:c1556-1209 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References