Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4401081..4401699 Replicon chromosome
Accession NZ_CP019250
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BWI84_RS22890 Protein ID WP_001291435.1
Coordinates 4401481..4401699 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BWI84_RS22885 Protein ID WP_000344800.1
Coordinates 4401081..4401455 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS22875 4396170..4397363 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BWI84_RS22880 4397386..4400535 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BWI84_RS22885 4401081..4401455 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BWI84_RS22890 4401481..4401699 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BWI84_RS22895 4401871..4402422 + 552 WP_000102564.1 maltose O-acetyltransferase -
BWI84_RS22900 4402538..4403008 + 471 WP_000136192.1 YlaC family protein -
BWI84_RS22905 4403172..4404722 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BWI84_RS22910 4404764..4405117 - 354 WP_000878141.1 DUF1428 family protein -
BWI84_RS22920 4405496..4405807 + 312 WP_000409911.1 MGMT family protein -
BWI84_RS22925 4405838..4406410 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T71988 WP_001291435.1 NZ_CP019250:4401481-4401699 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T71988 NZ_CP019250:4401481-4401699 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT71988 WP_000344800.1 NZ_CP019250:4401081-4401455 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT71988 NZ_CP019250:4401081-4401455 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References