Detailed information of TA system TA071977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2148920..2149545
• Replicon: chromosome
• Accession: NZ_CP019250
• Organism: Escherichia coli strain 13KWH46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWI84_RS11120
• Protein ID: WP_000911330.1
• Coordinates: 2149147..2149545 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BWI84_RS11115
• Protein ID: WP_000450524.1
• Coordinates: 2148920..2149147 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI84_RS11090	2144723..2145193	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
BWI84_RS11095	2145193..2145765	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BWI84_RS11100	2145911..2146789	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BWI84_RS11105	2146806..2147840	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
BWI84_RS11110	2148053..2148766	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BWI84_RS11115	2148920..2149147	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BWI84_RS11120	2149147..2149545	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BWI84_RS11125	2149692..2150555	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
BWI84_RS11130	2150570..2152585	+	2016	WP_063112794.1	tRNA cytosine(34) acetyltransferase TmcA	-
BWI84_RS11135	2152659..2153357	+	699	WP_000679823.1	esterase	-
BWI84_RS11140	2153467..2153667	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T71977 WP_000911330.1 NZ_CP019250:2149147-2149545 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T71977 NZ_CP019250:2149147-2149545 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT71977 WP_000450524.1 NZ_CP019250:2148920-2149147 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT71977 NZ_CP019250:2148920-2149147 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33