Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1715436..1716090 Replicon chromosome
Accession NZ_CP019250
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag BWI84_RS08950 Protein ID WP_063112724.1
Coordinates 1715683..1716090 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWI84_RS08945 Protein ID WP_000354046.1
Coordinates 1715436..1715702 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS08920 1710724..1711467 + 744 WP_000951964.1 SDR family oxidoreductase -
BWI84_RS08925 1711524..1712957 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
BWI84_RS08930 1713002..1713313 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
BWI84_RS08935 1713477..1714136 + 660 WP_000250274.1 hemolysin III family protein -
BWI84_RS08940 1714213..1715193 - 981 WP_001472148.1 tRNA-modifying protein YgfZ -
BWI84_RS08945 1715436..1715702 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWI84_RS08950 1715683..1716090 + 408 WP_063112724.1 protein YgfX Toxin
BWI84_RS08955 1716130..1716651 - 522 WP_001055874.1 flavodoxin FldB -
BWI84_RS08960 1716763..1717659 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BWI84_RS08965 1717684..1718394 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWI84_RS08970 1718400..1720133 + 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16082.04 Da        Isoelectric Point: 11.2511

>T71975 WP_063112724.1 NZ_CP019250:1715683-1716090 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLFHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T71975 NZ_CP019250:1715683-1716090 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGTTTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT71975 WP_000354046.1 NZ_CP019250:1715436-1715702 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT71975 NZ_CP019250:1715436-1715702 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References