Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1064559..1065190 Replicon chromosome
Accession NZ_CP019250
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag BWI84_RS05310 Protein ID WP_001259388.1
Coordinates 1064559..1064834 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag BWI84_RS05315 Protein ID WP_000593555.1
Coordinates 1064831..1065190 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS05295 1059563..1060630 + 1068 WP_000361485.1 HlyD family secretion protein -
BWI84_RS05300 1060627..1063362 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
BWI84_RS05305 1063362..1064486 + 1125 WP_001314210.1 ABC transporter permease -
BWI84_RS05310 1064559..1064834 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
BWI84_RS05315 1064831..1065190 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BWI84_RS05320 1065310..1065711 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
BWI84_RS05325 1065717..1066523 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
BWI84_RS05330 1066520..1067284 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
BWI84_RS05335 1067284..1068117 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
BWI84_RS05340 1068114..1069058 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1056960..1065190 8230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T71972 WP_001259388.1 NZ_CP019250:1064559-1064834 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T71972 NZ_CP019250:1064559-1064834 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT71972 WP_000593555.1 NZ_CP019250:1064831-1065190 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT71972 NZ_CP019250:1064831-1065190 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References