Detailed information of TA system TA071960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4103318..4103956
• Replicon: chromosome
• Accession: NZ_CP019245
• Organism: Escherichia coli strain Combat13F7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BWI83_RS20975
• Protein ID: WP_000813794.1
• Coordinates: 4103780..4103956 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWI83_RS20970
• Protein ID: WP_001270286.1
• Coordinates: 4103318..4103734 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI83_RS20950	4098470..4099411	-	942	WP_001251319.1	ABC transporter permease	-
BWI83_RS20955	4099412..4100425	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
BWI83_RS20960	4100443..4101588	-	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
BWI83_RS20965	4101830..4103239	-	1410	WP_088213419.1	PLP-dependent aminotransferase family protein	-
BWI83_RS20970	4103318..4103734	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BWI83_RS20975	4103780..4103956	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BWI83_RS20980	4104178..4104408	+	231	WP_000494244.1	YncJ family protein	-
BWI83_RS20985	4104500..4106461	-	1962	WP_024179169.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BWI83_RS20990	4106534..4107070	-	537	WP_088213418.1	DNA-binding transcriptional regulator SutR	-
BWI83_RS20995	4107123..4108352	+	1230	WP_071531711.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4108392..4109540	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T71960 WP_000813794.1 NZ_CP019245:c4103956-4103780 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T71960 NZ_CP019245:c4103956-4103780 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT71960 WP_001270286.1 NZ_CP019245:c4103734-4103318 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT71960 NZ_CP019245:c4103734-4103318 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB