Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2515202..2515856 Replicon chromosome
Accession NZ_CP019245
Organism Escherichia coli strain Combat13F7

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BWI83_RS12920 Protein ID WP_000244777.1
Coordinates 2515449..2515856 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWI83_RS12915 Protein ID WP_000354046.1
Coordinates 2515202..2515468 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI83_RS12890 2510371..2511114 + 744 WP_000951962.1 SDR family oxidoreductase -
BWI83_RS12895 2511171..2512604 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
BWI83_RS12900 2512649..2512960 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
BWI83_RS12905 2513124..2513783 + 660 WP_000250274.1 hemolysin III family protein -
BWI83_RS12910 2513979..2514959 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BWI83_RS12915 2515202..2515468 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWI83_RS12920 2515449..2515856 + 408 WP_000244777.1 toxin CptA Toxin
BWI83_RS12925 2515896..2516417 - 522 WP_001055874.1 flavodoxin FldB -
BWI83_RS12930 2516529..2517425 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BWI83_RS12935 2517450..2518160 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWI83_RS12940 2518166..2519899 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T71948 WP_000244777.1 NZ_CP019245:2515449-2515856 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T71948 NZ_CP019245:2515449-2515856 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT71948 WP_000354046.1 NZ_CP019245:2515202-2515468 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT71948 NZ_CP019245:2515202-2515468 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References