Detailed information of TA system TA071943

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1030788..1031491
• Replicon: chromosome
• Accession: NZ_CP019245
• Organism: Escherichia coli strain Combat13F7

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0L0H2I3
• Locus tag: BWI83_RS05330
• Protein ID: WP_021530985.1
• Coordinates: 1030788..1031129 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: BWI83_RS05335
• Protein ID: WP_126026844.1
• Coordinates: 1031150..1031491 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI83_RS05300	1026919..1027899	+	981	WP_148115926.1	sialate O-acetylesterase	-
BWI83_RS05305	1027907..1028557	-	651	WP_001037977.1	HNH endonuclease	-
BWI83_RS05325	1029530..1030537	-	1008	WP_004108748.1	restriction endonuclease	-
BWI83_RS05330	1030788..1031129	-	342	WP_021530985.1	TA system toxin CbtA family protein	Toxin
BWI83_RS05335	1031150..1031491	-	342	WP_126026844.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BWI83_RS05340	1031502..1032044	-	543	WP_126026845.1	DNA repair protein RadC	-
BWI83_RS05345	1032057..1032497	-	441	WP_126026846.1	antirestriction protein	-
BWI83_RS05350	1032528..1033349	-	822	WP_148115927.1	DUF945 domain-containing protein	-
BWI83_RS05355	1033470..1033943	-	474	WP_126026848.1	hypothetical protein	-
BWI83_RS05360	1034015..1034467	-	453	WP_031280325.1	hypothetical protein	-
BWI83_RS05365	1034503..1035219	-	717	WP_001757887.1	WYL domain-containing protein	-
BWI83_RS05370	1035463..1036338	-	876	WP_126026849.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	1021880..1051558	29678

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12838.71 Da
• Isoelectric Point: 6.4789

>T71943 WP_021530985.1 NZ_CP019245:c1031129-1030788 [Escherichia coli]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T71943 NZ_CP019245:c1031129-1030788 [Escherichia coli]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACAGGTTTATTGAAAGCGCG
GCACGGTGAACATTGGGAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12724.40 Da
• Isoelectric Point: 6.7345

>AT71943 WP_126026844.1 NZ_CP019245:c1031491-1031150 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVIPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT71943 NZ_CP019245:c1031491-1031150 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGATACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATTAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0L0H2I3