Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 2321..2868 Replicon plasmid pCombat2C1-1
Accession NZ_CP019244
Organism Escherichia coli strain Combat2C1

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag BWI82_RS26865 Protein ID WP_000969996.1
Coordinates 2587..2868 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A723YP93
Locus tag BWI82_RS26860 Protein ID WP_021559054.1
Coordinates 2321..2590 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI82_RS26845 1..261 + 261 WP_000083819.1 replication regulatory protein RepA -
BWI82_RS27810 485..547 + 63 Protein_1 RepA leader peptide Tap -
BWI82_RS26850 551..1408 + 858 WP_032495133.1 incFII family plasmid replication initiator RepA -
BWI82_RS27815 2108..2266 + 159 WP_072649723.1 hypothetical protein -
BWI82_RS26860 2321..2590 + 270 WP_021559054.1 hypothetical protein Antitoxin
BWI82_RS26865 2587..2868 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWI82_RS27820 2903..2965 + 63 Protein_6 3'-5' exonuclease -
BWI82_RS26870 3054..3338 + 285 WP_001407321.1 ribbon-helix-helix domain-containing protein -
BWI82_RS26875 3338..3615 + 278 Protein_8 type II toxin-antitoxin system RelE/ParE family toxin -
BWI82_RS26880 3711..3968 + 258 WP_001685860.1 hypothetical protein -
BWI82_RS26885 3970..4677 + 708 WP_058100829.1 hypothetical protein -
BWI82_RS26890 4681..4971 + 291 WP_148116751.1 hypothetical protein -
BWI82_RS26895 5008..5712 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
BWI82_RS26905 5814..6293 + 480 WP_000888203.1 Rrf2 family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid oqxB / oqxA - 1..71430 71430


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T71936 WP_000969996.1 NZ_CP019244:2587-2868 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T71936 NZ_CP019244:2587-2868 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10222.60 Da        Isoelectric Point: 8.8735

>AT71936 WP_021559054.1 NZ_CP019244:2321-2590 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT71936 NZ_CP019244:2321-2590 [Escherichia coli]
ATGGCACAGATTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGCCTCTCAGTTAATCCGGGATTTTATGCGCCAGACTGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A723YP93

References