Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4472869..4473487 Replicon chromosome
Accession NZ_CP019243
Organism Escherichia coli strain Combat2C1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BWI82_RS22895 Protein ID WP_001291435.1
Coordinates 4472869..4473087 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BWI82_RS22900 Protein ID WP_000344800.1
Coordinates 4473113..4473487 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI82_RS22860 4468156..4468728 + 573 WP_000779830.1 YbaY family lipoprotein -
BWI82_RS22865 4468759..4469070 - 312 WP_000409908.1 MGMT family protein -
BWI82_RS22875 4469449..4469802 + 354 WP_000878138.1 DUF1428 family protein -
BWI82_RS22880 4469844..4471394 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BWI82_RS22885 4471558..4472028 - 471 WP_000136192.1 YlaC family protein -
BWI82_RS22890 4472144..4472695 - 552 WP_000102539.1 maltose O-acetyltransferase -
BWI82_RS22895 4472869..4473087 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BWI82_RS22900 4473113..4473487 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BWI82_RS22905 4474032..4477181 - 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
BWI82_RS22910 4477204..4478397 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T71934 WP_001291435.1 NZ_CP019243:c4473087-4472869 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T71934 NZ_CP019243:c4473087-4472869 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT71934 WP_000344800.1 NZ_CP019243:c4473487-4473113 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT71934 NZ_CP019243:c4473487-4473113 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References