Detailed information of TA system TA071933

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4283925..4284760
• Replicon: chromosome
• Accession: NZ_CP019243
• Organism: Escherichia coli strain Combat2C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: BWI82_RS21915
• Protein ID: WP_000854700.1
• Coordinates: 4284383..4284760 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: BWI82_RS21910
• Protein ID: WP_001278232.1
• Coordinates: 4283925..4284293 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI82_RS21890	4281585..4282403	+	819	WP_001234709.1	DUF945 domain-containing protein	-
BWI82_RS21895	4282495..4282980	+	486	WP_000213723.1	antirestriction protein	-
BWI82_RS21900	4282996..4283472	+	477	WP_001186199.1	RadC family protein	-
BWI82_RS21905	4283541..4283762	+	222	WP_000692353.1	DUF987 domain-containing protein	-
BWI82_RS21910	4283925..4284293	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BWI82_RS21915	4284383..4284760	+	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
BWI82_RS21920	4284757..4285245	+	489	WP_000761726.1	hypothetical protein	-
BWI82_RS21925	4285257..4285454	+	198	WP_000839247.1	DUF957 domain-containing protein	-
BWI82_RS21930	4285539..4286393	+	855	WP_024174313.1	DUF4942 domain-containing protein	-
BWI82_RS21935	4286486..4287805	-	1320	WP_000144686.1	site-specific integrase	-
BWI82_RS21940	4288171..4288509	+	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
BWI82_RS21945	4288944..4289438	-	495	WP_001059463.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	4258192..4333739	75547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T71933 WP_000854700.1 NZ_CP019243:4284383-4284760 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T71933 NZ_CP019243:4284383-4284760 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT71933 WP_001278232.1 NZ_CP019243:4283925-4284293 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71933 NZ_CP019243:4283925-4284293 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2

Antitoxin

• AlphaFold DB: E3PJ72