Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3969649..3970199 Replicon chromosome
Accession NZ_CP019243
Organism Escherichia coli strain Combat2C1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag BWI82_RS20360 Protein ID WP_001160966.1
Coordinates 3969885..3970199 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag BWI82_RS20355 Protein ID WP_000125568.1
Coordinates 3969649..3969882 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI82_RS20330 3964722..3965009 + 288 WP_000203741.1 ferredoxin-like protein FixX -
BWI82_RS20335 3965068..3966399 + 1332 WP_001183198.1 MFS transporter -
BWI82_RS20340 3966507..3967037 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BWI82_RS20345 3967030..3968892 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
BWI82_RS20350 3969084..3969563 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
BWI82_RS20355 3969649..3969882 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BWI82_RS20360 3969885..3970199 + 315 WP_001160966.1 CcdB family protein Toxin
BWI82_RS20365 3970196..3971044 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BWI82_RS20370 3971051..3971428 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BWI82_RS20375 3971431..3972252 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BWI82_RS20380 3972249..3973238 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BWI82_RS20385 3973238..3974524 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T71931 WP_001160966.1 NZ_CP019243:3969885-3970199 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T71931 NZ_CP019243:3969885-3970199 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT71931 WP_000125568.1 NZ_CP019243:3969649-3969882 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT71931 NZ_CP019243:3969649-3969882 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References