Detailed information of TA system TA071918

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2153744..2154576
• Replicon: chromosome
• Accession: NZ_CP019243
• Organism: Escherichia coli strain Combat2C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: BWI82_RS11115
• Protein ID: WP_000854753.1
• Coordinates: 2154202..2154576 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BWI82_RS11110
• Protein ID: WP_001531802.1
• Coordinates: 2153744..2154112 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI82_RS11075	2150578..2151033	+	456	WP_000581502.1	hypothetical protein	-
BWI82_RS27720	2151152..2151363	-	212	Protein_2032	hypothetical protein	-
BWI82_RS11090	2151444..2152265	+	822	WP_001531953.1	DUF945 domain-containing protein	-
BWI82_RS11095	2152320..2152805	+	486	WP_001531954.1	antirestriction protein	-
BWI82_RS11100	2152821..2153297	+	477	WP_001186726.1	RadC family protein	-
BWI82_RS11105	2153360..2153581	+	222	WP_000692329.1	DUF987 domain-containing protein	-
BWI82_RS11110	2153744..2154112	+	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BWI82_RS11115	2154202..2154576	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
BWI82_RS11120	2154573..2155061	+	489	WP_000777541.1	hypothetical protein	-
BWI82_RS11125	2155073..2155270	+	198	WP_000839281.1	DUF957 domain-containing protein	-
BWI82_RS11130	2155367..2155936	+	570	WP_001290247.1	DUF4942 domain-containing protein	-
BWI82_RS11135	2156278..2156448	+	171	Protein_2042	IS110 family transposase	-
BWI82_RS11140	2157258..2158241	+	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
BWI82_RS11145	2158313..2159461	+	1149	WP_000905914.1	capsule polysaccharide transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2156344..2156448	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T71918 WP_000854753.1 NZ_CP019243:2154202-2154576 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T71918 NZ_CP019243:2154202-2154576 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT71918 WP_001531802.1 NZ_CP019243:2153744-2154112 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71918 NZ_CP019243:2153744-2154112 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0