Detailed information of TA system TA071898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2876461..2877002
• Replicon: chromosome
• Accession: NZ_CP019240
• Organism: Rhodoferax antarcticus strain DSMZ24876

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: RA876_RS13875
• Protein ID: WP_075588080.1
• Coordinates: 2876679..2877002 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: RA876_RS13870
• Protein ID: WP_075588079.1
• Coordinates: 2876461..2876679 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RA876_RS13850	2871905..2874037	+	2133	WP_075588075.1	methionine--tRNA ligase	-
RA876_RS13855	2874208..2874663	+	456	WP_075588076.1	fasciclin domain-containing protein	-
RA876_RS13860	2874726..2875337	+	612	WP_083634068.1	heme-binding protein	-
RA876_RS13865	2875763..2876216	+	454	Protein_2710	cation:proton antiporter	-
RA876_RS13870	2876461..2876679	+	219	WP_075588079.1	antitoxin MazE family protein	Antitoxin
RA876_RS13875	2876679..2877002	+	324	WP_075588080.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RA876_RS13880	2877072..2877596	-	525	WP_075588134.1	NADH-quinone oxidoreductase subunit B family protein	-
RA876_RS13885	2877610..2879196	-	1587	WP_075588081.1	NADH-quinone oxidoreductase subunit C	-
RA876_RS13890	2879201..2880655	-	1455	WP_075588082.1	hydrogenase 4 subunit F	-
RA876_RS13895	2880652..2881317	-	666	WP_075588083.1	formate hydrogenlyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11453.51 Da
• Isoelectric Point: 8.0424

>T71898 WP_075588080.1 NZ_CP019240:2876679-2877002 [Rhodoferax antarcticus]
MRGDFVTIAMQGDFGKPRPALVIQADPYDEHTSVTVLPVTAALVAAPLLRITIEPSPKNGLQKPSQVMVDKAMTVKRDKV
GPAFGHIDAAAMIEVERCLAVFLGIAK

Nucleotide

• Length: 324 bp

>T71898 NZ_CP019240:2876679-2877002 [Rhodoferax antarcticus]
ATGCGTGGCGACTTTGTGACCATCGCTATGCAAGGCGACTTTGGCAAGCCCAGACCCGCCTTGGTGATTCAGGCTGACCC
GTACGACGAGCACACCAGTGTCACCGTTCTGCCCGTGACAGCCGCGCTGGTTGCCGCGCCGCTATTGCGCATCACTATTG
AGCCAAGTCCAAAAAACGGTTTGCAAAAGCCGTCACAGGTGATGGTAGATAAAGCGATGACCGTGAAGCGCGACAAGGTT
GGCCCAGCCTTCGGGCACATTGACGCTGCAGCCATGATCGAGGTTGAGCGTTGTCTTGCTGTATTTTTGGGCATCGCCAA
ATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8364.60 Da
• Isoelectric Point: 6.9457

>AT71898 WP_075588079.1 NZ_CP019240:2876461-2876679 [Rhodoferax antarcticus]
MLHVNLRVQKYRDALRMAGLRPVQIWVPDTRTPDFAQECRRQCRLVAQADMADQNMQTFMNEALADVDGWTA

Nucleotide

• Length: 219 bp

>AT71898 NZ_CP019240:2876461-2876679 [Rhodoferax antarcticus]
ATGCTGCATGTCAATTTACGTGTTCAAAAGTACCGCGATGCGCTTCGTATGGCGGGGTTGCGCCCTGTGCAAATCTGGGT
GCCAGATACGCGAACGCCAGACTTCGCTCAGGAATGCCGTCGCCAGTGCCGCCTTGTCGCGCAAGCGGACATGGCCGACC
AGAACATGCAGACATTCATGAATGAAGCCTTAGCAGATGTGGACGGCTGGACGGCTTAA