Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 6232..6767 Replicon plasmid unnamed2
Accession NZ_CP019238
Organism Rhodoferax koreense strain DCY-110

Toxin (Protein)


Gene name mazF Uniprot ID A0A7W6J9K5
Locus tag RD110_RS26980 Protein ID WP_021561444.1
Coordinates 6232..6552 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7W6J9M0
Locus tag RD110_RS26985 Protein ID WP_021561443.1
Coordinates 6552..6767 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RD110_RS26940 1593..2375 - 783 Protein_3 hypothetical protein -
RD110_RS26945 2446..3041 - 596 Protein_4 undecaprenyl-diphosphatase -
RD110_RS26950 3098..3433 - 336 WP_005797945.1 PepSY domain-containing protein -
RD110_RS26975 5433..5996 - 564 WP_021561445.1 recombinase family protein -
RD110_RS26980 6232..6552 - 321 WP_021561444.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RD110_RS26985 6552..6767 - 216 WP_021561443.1 antitoxin MazE family protein Antitoxin
RD110_RS26990 7152..7811 + 660 WP_011178412.1 recombinase family protein -
RD110_RS26995 7813..8487 - 675 WP_011178411.1 DNA methylase -
RD110_RS27000 8642..9073 - 432 WP_001665065.1 OmpA family protein -
RD110_RS27005 9089..9787 - 699 WP_011114053.1 conjugal transfer protein TraX -
RD110_RS27010 9787..10053 - 267 WP_010890140.1 hypothetical protein -
RD110_RS27015 10082..10717 - 636 WP_011114052.1 transglycosylase SLT domain-containing protein -
RD110_RS27020 10731..11318 - 588 WP_010890138.1 conjugal transfer protein TrbM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..71276 71276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin MazE-like

(7-70)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11585.51 Da        Isoelectric Point: 6.2890

>T71885 WP_021561444.1 NZ_CP019238:c6552-6232 [Rhodoferax koreense]
MQRGDLVTVSLQGDYGKPRPALIVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Download         Length: 321 bp

>T71885 NZ_CP019238:c6552-6232 [Rhodoferax koreense]
ATGCAGCGAGGCGACCTCGTGACGGTTTCATTGCAGGGTGACTACGGCAAACCACGACCAGCCCTGATCGTTCAATCAGA
CCTACTAACCGACTTGGAAAGCGTTGTGCTGTGCCCAGTGACAAGCGACCTGCGCAATGCCGCGTTTCGCGTCACCGTGG
AACCGAATCCAGCTAACGGGCTGCGCGCACTATCGCAAGTTATGGTGGACAAGCTCTCGACGCTACCGCGCACCAAAATC
AGTGAACCGTTTGGCCGCCTCGACGATGAGCGAATGAAAGCAATAGATAGGGCGCTCCTGTTGGTCGTTGGCGTTATTTG
A

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8159.19 Da        Isoelectric Point: 7.2801

>AT71885 WP_021561443.1 NZ_CP019238:c6767-6552 [Rhodoferax koreense]
MPSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFTAQVRKQCQSLKGDPAEAEVLRFTEEAASHVEGWE

Download         Length: 216 bp

>AT71885 NZ_CP019238:c6767-6552 [Rhodoferax koreense]
ATGCCATCAATTCACGAATCAAAGGTAGCCCGACACCGCGAACGGATGCGCGCGGCGGGGCTGCGGCCTGTGCAGTTTTG
GGTGCCGGACACCCGTTCGCCTGAGTTCACGGCGCAAGTGCGCAAGCAATGCCAAAGCCTGAAGGGCGACCCAGCAGAAG
CAGAGGTTTTGCGCTTCACCGAAGAGGCGGCATCGCACGTTGAGGGCTGGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W6J9K5

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6J9M0

References