Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5092382..5093024 Replicon chromosome
Accession NZ_CP019230
Organism Bacillus thuringiensis strain YGd22-03

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BVH75_RS27430 Protein ID WP_000635965.1
Coordinates 5092674..5093024 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BVH75_RS27425 Protein ID WP_000004570.1
Coordinates 5092382..5092669 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVH75_RS27400 5087699..5088661 + 963 WP_087876037.1 UV DNA damage repair endonuclease UvsE -
BVH75_RS27405 5088654..5089226 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BVH75_RS27410 5089320..5089679 + 360 WP_000583417.1 holo-ACP synthase -
BVH75_RS27415 5089836..5090786 + 951 WP_153043809.1 outer membrane lipoprotein carrier protein LolA -
BVH75_RS27420 5090904..5092073 + 1170 WP_000390617.1 alanine racemase -
BVH75_RS27425 5092382..5092669 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BVH75_RS27430 5092674..5093024 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BVH75_RS27435 5093092..5095260 + 2169 WP_000426241.1 RNA-binding transcriptional accessory protein -
BVH75_RS27440 5095318..5095434 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BVH75_RS27445 5095630..5096088 + 459 WP_000344235.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T71882 WP_000635965.1 NZ_CP019230:5092674-5093024 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T71882 NZ_CP019230:5092674-5093024 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT71882 WP_000004570.1 NZ_CP019230:5092382-5092669 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT71882 NZ_CP019230:5092382-5092669 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References