Detailed information of TA system TA071872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 10342..10883
• Replicon: plasmid pXOO43
• Accession: NZ_CP019227
• Organism: Xanthomonas oryzae pv. oryzae strain IX-280

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BCR61_RS24675
• Protein ID: WP_015462614.1
• Coordinates: 10342..10668 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: BCR61_RS24680
• Protein ID: WP_003491150.1
• Coordinates: 10665..10883 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCR61_RS25280	5533..5706	+	174	WP_162886871.1	hypothetical protein	-
BCR61_RS24665	5710..7284	+	1575	WP_039587421.1	type IV secretion system DNA-binding domain-containing protein	-
BCR61_RS24670	7298..10273	+	2976	WP_075244571.1	conjugative relaxase	-
BCR61_RS24675	10342..10668	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCR61_RS24680	10665..10883	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
BCR61_RS24685	11021..11407	-	387	WP_075244572.1	hypothetical protein	-
BCR61_RS24690	11484..11903	-	420	WP_047340369.1	hypothetical protein	-
BCR61_RS24695	12028..12906	-	879	WP_075244574.1	lytic transglycosylase domain-containing protein	-
BCR61_RS24700	12917..13939	-	1023	WP_075244575.1	P-type DNA transfer ATPase VirB11	-
BCR61_RS24705	13950..15170	-	1221	WP_075244576.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42975	42975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T71872 WP_015462614.1 NZ_CP019227:c10668-10342 [Xanthomonas oryzae pv. oryzae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T71872 NZ_CP019227:c10668-10342 [Xanthomonas oryzae pv. oryzae]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT71872 WP_003491150.1 NZ_CP019227:c10883-10665 [Xanthomonas oryzae pv. oryzae]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT71872 NZ_CP019227:c10883-10665 [Xanthomonas oryzae pv. oryzae]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7