Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 45079..45761 Replicon plasmid pDHQP20152271_1
Accession NZ_CP019224
Organism Mycobacterium intracellulare subsp. chimaera strain CDC 2015-22-71 isolate PA-Patient

Toxin (Protein)


Gene name vapC Uniprot ID A0A854IDQ2
Locus tag BWK49_RS28940 Protein ID WP_040624374.1
Coordinates 45079..45495 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID D5P579
Locus tag BWK49_RS28945 Protein ID WP_007172349.1
Coordinates 45498..45761 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWK49_RS28920 40846..40984 - 139 Protein_49 type II toxin-antitoxin system VapC family toxin -
BWK49_RS28925 41090..42742 - 1653 WP_040624340.1 IS607 family element transposase accessory protein TnpB -
BWK49_RS28930 42742..43323 - 582 WP_007172352.1 IS607 family transposase -
BWK49_RS28935 43466..44797 + 1332 WP_023363507.1 IS256 family transposase -
BWK49_RS28940 45079..45495 - 417 WP_040624374.1 type II toxin-antitoxin system VapC family toxin Toxin
BWK49_RS28945 45498..45761 - 264 WP_007172349.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BWK49_RS28950 45974..46837 + 864 WP_142383462.1 hypothetical protein -
BWK49_RS28955 46901..48004 + 1104 WP_051404593.1 hypothetical protein -
BWK49_RS28960 48090..49130 + 1041 WP_023363502.1 C40 family peptidase -
BWK49_RS28965 49244..50689 + 1446 WP_101930849.1 type VII secretion protein EccB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - esxN / eccA5 1..97268 97268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(1-82)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14879.84 Da        Isoelectric Point: 5.4519

>T71867 WP_040624374.1 NZ_CP019224:c45495-45079 [Mycobacterium intracellulare subsp. chimaera]
MIIDSSAIVALIQGEDPYTEQIAAAIAADPSPVMSTANAAECLIVLTNRHGATARTVFDRLRSEINLGFQPFTLEHAIAA
HRAYLHYGKGRHPAALNYGDTMAYATAKLAHEPLIAIGNDFTQTDLEFNGVIGYWPAP

Download         Length: 417 bp

>T71867 NZ_CP019224:c45495-45079 [Mycobacterium intracellulare subsp. chimaera]
CTGATCATCGACTCTTCCGCGATCGTCGCCCTCATCCAGGGCGAGGACCCCTACACCGAACAGATCGCGGCCGCCATCGC
CGCCGATCCCAGCCCCGTCATGTCCACGGCCAACGCAGCCGAATGCCTCATCGTTCTCACCAACCGACACGGCGCGACCG
CGCGCACCGTCTTCGACCGCCTACGATCCGAAATCAACCTCGGCTTCCAGCCCTTCACCCTCGAACACGCGATCGCCGCC
CACCGCGCCTACCTCCACTACGGAAAAGGCCGCCACCCCGCCGCCCTCAACTACGGCGACACCATGGCCTACGCCACCGC
CAAACTCGCACACGAACCCCTCATCGCCATCGGCAACGACTTCACCCAAACCGACCTCGAATTCAACGGCGTCATCGGCT
ACTGGCCAGCGCCCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9884.20 Da        Isoelectric Point: 4.8359

>AT71867 WP_007172349.1 NZ_CP019224:c45761-45498 [Mycobacterium intracellulare subsp. chimaera]
MAFNVKDEEVIRFADELAARLHLPSRIDAIRYALRAQIEVTQSRTANRSAELLDVLKTEIWPLLDDRSPITKTEREQALG
YDTATGV

Download         Length: 264 bp

>AT71867 NZ_CP019224:c45761-45498 [Mycobacterium intracellulare subsp. chimaera]
ATGGCTTTCAACGTGAAAGACGAAGAGGTCATCCGCTTCGCAGACGAGCTCGCCGCGCGCCTCCACCTCCCCAGCCGCAT
CGACGCCATCCGCTACGCCCTGCGAGCCCAGATCGAAGTCACCCAATCCCGCACCGCAAACCGCTCCGCAGAACTGCTCG
ACGTCCTCAAAACCGAGATCTGGCCACTACTGGACGACCGATCCCCAATCACCAAAACCGAACGCGAACAAGCCCTCGGA
TACGACACAGCAACGGGAGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854IDQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y0TBQ2

References