Detailed information of TA system TA071864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 19816..20372
• Replicon: plasmid pDHQP20152271_2
• Accession: NZ_CP019223
• Organism: Mycobacterium intracellulare subsp. chimaera strain CDC 2015-22-71 isolate PA-Patient

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BWK49_RS28645
• Protein ID: WP_046182242.1
• Coordinates: 20097..20372 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BWK49_RS28640
• Protein ID: WP_082122855.1
• Coordinates: 19816..20100 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWK49_RS28620	14863..15117	-	255	WP_142383129.1	antitoxin VbhA family protein	-
BWK49_RS28625	15353..18187	+	2835	WP_054585406.1	relaxase domain-containing protein	-
BWK49_RS28630	18332..19129	+	798	WP_046182240.1	sigma 54 modulation/S30EA ribosomal C-terminal domain-containing protein	-
BWK49_RS28635	19198..19587	+	390	Protein_20	pyridoxamine 5'-phosphate oxidase family protein	-
BWK49_RS28640	19816..20100	+	285	WP_082122855.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWK49_RS28645	20097..20372	+	276	WP_046182242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWK49_RS28650	20490..20705	-	216	WP_046182243.1	hypothetical protein	-
BWK49_RS28655	20782..21057	+	276	WP_046182244.1	hypothetical protein	-
BWK49_RS28660	21206..21772	+	567	WP_046182262.1	recombinase family protein	-
BWK49_RS29990	22248..23000	+	753	WP_172417609.1	1-aminocyclopropane-1-carboxylate synthase	-
BWK49_RS28675	23234..24190	+	957	WP_082122856.1	fatty acid desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39887	39887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10459.92 Da
• Isoelectric Point: 10.0890

>T71864 WP_046182242.1 NZ_CP019223:20097-20372 [Mycobacterium intracellulare subsp. chimaera]
VTDRRYELIIAPTAKKQLAEQLPESAAFAAYEFIIGPLLDNPHRVGKQLRPPLHDRHSARRGTYRVLYRIDDERQQVTVV
GVFSRADAYRA

Nucleotide

• Length: 276 bp

>T71864 NZ_CP019223:20097-20372 [Mycobacterium intracellulare subsp. chimaera]
GTGACCGACCGACGATATGAGCTGATAATCGCTCCGACCGCGAAAAAACAACTGGCCGAACAACTTCCCGAATCGGCGGC
GTTCGCTGCCTATGAGTTCATCATCGGTCCACTGCTCGACAATCCCCACCGCGTGGGCAAGCAGCTCCGACCACCGTTGC
ACGATCGCCATAGCGCACGCCGCGGCACCTACCGCGTGCTCTACAGAATTGACGACGAGCGTCAGCAAGTCACCGTTGTT
GGCGTGTTCAGCCGCGCGGACGCATACCGTGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9876.02 Da
• Isoelectric Point: 4.4269

>AT71864 WP_082122855.1 NZ_CP019223:19816-20100 [Mycobacterium intracellulare subsp. chimaera]
MSEVSSLAEAKAHLSELVARVGDQHERITVTVHGRPAAVLLAVDDLESLEETIAVLSDAAALRALAEADAELARGEGENQ
DSLSAAMQARRAKT

Nucleotide

• Length: 285 bp

>AT71864 NZ_CP019223:19816-20100 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTGAAGTCAGCTCACTCGCCGAGGCGAAGGCGCATCTATCGGAACTGGTGGCCCGTGTCGGCGATCAGCACGAGCG
GATCACGGTAACGGTGCATGGACGCCCTGCGGCGGTCCTTCTTGCCGTCGACGATCTGGAGTCACTCGAGGAAACTATTG
CGGTGCTGTCCGATGCGGCGGCGCTCCGTGCCCTTGCCGAGGCCGATGCTGAGCTAGCCCGGGGGGAAGGGGAGAACCAG
GACAGCCTTTCCGCAGCGATGCAAGCACGCCGAGCTAAGACGTGA