Detailed information of TA system TA071861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2615300..2615964
• Replicon: chromosome
• Accession: NZ_CP019221
• Organism: Mycobacterium intracellulare subsp. chimaera strain CDC 2015-22-71 isolate PA-Patient

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: BWK49_RS12295
• Protein ID: WP_009954498.1
• Coordinates: 2615557..2615964 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BWK49_RS12290
• Protein ID: WP_009954496.1
• Coordinates: 2615300..2615560 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWK49_RS12265	2611809..2612300	-	492	WP_014711439.1	DUF1707 domain-containing protein	-
BWK49_RS12270	2612370..2612966	+	597	WP_042911492.1	TetR/AcrR family transcriptional regulator	-
BWK49_RS12275	2612971..2614239	-	1269	WP_042911493.1	cytochrome P450	-
BWK49_RS12280	2614397..2614849	+	453	WP_042911495.1	VOC family protein	-
BWK49_RS12285	2614920..2615177	+	258	WP_042911496.1	hypothetical protein	-
BWK49_RS12290	2615300..2615560	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWK49_RS12295	2615557..2615964	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWK49_RS12300	2616142..2619243	+	3102	WP_042911498.1	DEAD/DEAH box helicase	-
BWK49_RS12305	2619305..2620870	-	1566	WP_042911499.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T71861 WP_009954498.1 NZ_CP019221:2615557-2615964 [Mycobacterium intracellulare subsp. chimaera]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T71861 NZ_CP019221:2615557-2615964 [Mycobacterium intracellulare subsp. chimaera]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACTACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGGTCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCTGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT71861 WP_009954496.1 NZ_CP019221:2615300-2615560 [Mycobacterium intracellulare subsp. chimaera]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT71861 NZ_CP019221:2615300-2615560 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: J9WD27