Detailed information of TA system TA071858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1601954..1602498
• Replicon: chromosome
• Accession: NZ_CP019221
• Organism: Mycobacterium intracellulare subsp. chimaera strain CDC 2015-22-71 isolate PA-Patient

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A854HUL3
• Locus tag: BWK49_RS07905
• Protein ID: WP_042910934.1
• Coordinates: 1602253..1602498 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S4Z4W6
• Locus tag: BWK49_RS07900
• Protein ID: WP_020821851.1
• Coordinates: 1601954..1602238 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWK49_RS07875	1597028..1597627	-	600	WP_009953444.1	lipid droplet-associated protein	-
BWK49_RS07880	1597717..1598715	+	999	WP_042910931.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BWK49_RS07885	1598761..1599906	-	1146	WP_179945068.1	hypothetical protein	-
BWK49_RS07890	1600036..1601109	+	1074	WP_042910932.1	redox-regulated ATPase YchF	-
BWK49_RS07895	1601126..1601866	+	741	WP_042910933.1	aminoglycoside 3'-phosphotransferase	-
BWK49_RS07900	1601954..1602238	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWK49_RS07905	1602253..1602498	+	246	WP_042910934.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWK49_RS07910	1602534..1603085	+	552	WP_036454798.1	VOC family protein	-
BWK49_RS07915	1603164..1603562	+	399	WP_036454800.1	VOC family protein	-
BWK49_RS07920	1603657..1603854	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	-
BWK49_RS07925	1603851..1604219	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	-
BWK49_RS07930	1604402..1604743	+	342	WP_172417493.1	hypothetical protein	-
BWK49_RS29835	1604829..1605188	+	360	WP_042910935.1	hypothetical protein	-
BWK49_RS07940	1605373..1605948	+	576	WP_075630262.1	C40 family peptidase	-
BWK49_RS07945	1606311..1606634	+	324	WP_036454806.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (29-80)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9380.95 Da
• Isoelectric Point: 11.2612

>T71858 WP_042910934.1 NZ_CP019221:1602253-1602498 [Mycobacterium intracellulare subsp. chimaera]
MTAAAKRALTHLLPESVAIACWEFIRGPLAENPQRVGKPLRGQLEGRYSARRGEFRVIYRIFDERVVVPVIHIAHRRDVY
R

Nucleotide

• Length: 246 bp

>T71858 NZ_CP019221:1602253-1602498 [Mycobacterium intracellulare subsp. chimaera]
ATGACGGCAGCTGCGAAGAGGGCGCTCACTCATTTGTTGCCCGAGTCGGTGGCCATTGCTTGCTGGGAGTTCATTCGGGG
TCCGCTCGCGGAGAACCCGCAGCGGGTGGGCAAGCCGCTTCGCGGCCAGTTGGAAGGGCGATACTCGGCGCGCAGGGGCG
AGTTTCGCGTCATTTACCGGATCTTCGACGAACGGGTGGTGGTCCCCGTCATCCACATCGCGCATCGCCGCGACGTTTAC
CGGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10513.88 Da
• Isoelectric Point: 4.3705

>AT71858 WP_020821851.1 NZ_CP019221:1601954-1602238 [Mycobacterium intracellulare subsp. chimaera]
MSTLPLAEVRANLSKLVDEAVRTHERIEVTRQGRRAAVILSADDFDSIMETLAILSDQDLMREVRAAESEAEDGALYTLD
QVTEEMRAVGRLPR

Nucleotide

• Length: 285 bp

>AT71858 NZ_CP019221:1601954-1602238 [Mycobacterium intracellulare subsp. chimaera]
ATGTCGACGCTTCCGCTGGCCGAGGTCCGAGCCAACCTTTCCAAGCTGGTCGACGAGGCCGTCCGGACCCATGAGCGCAT
CGAGGTCACCAGGCAAGGTCGCCGCGCGGCGGTGATTCTCAGCGCGGATGACTTCGATTCGATTATGGAAACTCTGGCCA
TCCTCAGCGATCAAGACTTGATGCGCGAAGTCCGAGCGGCAGAGTCAGAGGCAGAGGACGGCGCGCTCTACACACTCGAT
CAGGTGACCGAGGAGATGCGTGCTGTCGGCCGGCTCCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A854HUL3

Antitoxin

• AlphaFold DB: S4Z4W6