Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 1073737..1074404 Replicon chromosome
Accession NZ_CP019221
Organism Mycobacterium intracellulare subsp. chimaera strain CDC 2015-22-71 isolate PA-Patient

Toxin (Protein)


Gene name vapC Uniprot ID D5PE24
Locus tag BWK49_RS05290 Protein ID WP_007168407.1
Coordinates 1074012..1074404 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID D5PE23
Locus tag BWK49_RS05285 Protein ID WP_007168406.1
Coordinates 1073737..1074012 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWK49_RS29795 1069241..1069567 - 327 WP_033711234.1 hypothetical protein -
BWK49_RS05260 1069557..1069790 - 234 Protein_1033 transposase -
BWK49_RS05265 1069808..1071019 - 1212 Protein_1034 ISL3 family transposase -
BWK49_RS05270 1071158..1071790 - 633 Protein_1035 DDE-type integrase/transposase/recombinase -
BWK49_RS29340 1071742..1071882 - 141 WP_145958858.1 SDR family oxidoreductase -
BWK49_RS05275 1071926..1072629 - 704 Protein_1037 histidine phosphatase family protein -
BWK49_RS05280 1072712..1073584 + 873 WP_007168405.1 MaoC family dehydratase N-terminal domain-containing protein -
BWK49_RS05285 1073737..1074012 + 276 WP_007168406.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BWK49_RS05290 1074012..1074404 + 393 WP_007168407.1 type II toxin-antitoxin system VapC family toxin Toxin
BWK49_RS05295 1074552..1075711 - 1160 Protein_1041 trehalose-phosphatase -
BWK49_RS05300 1075753..1077091 - 1339 Protein_1042 DUF1298 domain-containing protein -
BWK49_RS29345 1077353..1077475 - 123 Protein_1043 IS1595 family transposase -
BWK49_RS05310 1077811..1079145 + 1335 Protein_1044 IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-119)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-45)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14305.73 Da        Isoelectric Point: 10.5032

>T71855 WP_007168407.1 NZ_CP019221:1074012-1074404 [Mycobacterium intracellulare subsp. chimaera]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRLPRVSSALARTEVMRALLHKGESARRAGRRALASLDLLRIDNRLLDLAG
GLLPIELRTLDAIHLATAQRLGMDLGRLCTYDDRMRDAAEALGMAVIAPS

Download         Length: 393 bp

>T71855 NZ_CP019221:1074012-1074404 [Mycobacterium intracellulare subsp. chimaera]
ATGGCGGCCATCTACCTGGACTCGTCCGCAATCGTCAAGCTTGCAGTTCGTGAGCCAGAGTCGGATGCGCTGCGTCGCTA
CCTTCGCACCCGGCTGCCGCGGGTGTCGAGTGCCCTTGCGCGGACGGAGGTCATGCGGGCTCTGCTCCACAAGGGTGAAT
CAGCCCGAAGAGCCGGACGCCGGGCGCTCGCGAGCCTAGACCTACTACGCATCGACAACCGATTGCTCGATCTTGCTGGT
GGGCTGCTGCCAATCGAGCTACGCACCCTCGATGCAATTCACCTGGCGACTGCCCAGCGGCTCGGCATGGATCTTGGCCG
GTTGTGCACTTACGACGACCGCATGCGCGACGCCGCCGAGGCGCTCGGCATGGCAGTGATTGCCCCGTCTTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10150.63 Da        Isoelectric Point: 5.1881

>AT71855 WP_007168406.1 NZ_CP019221:1073737-1074012 [Mycobacterium intracellulare subsp. chimaera]
MSTVGVRELRQRASELLRRVEAGETIEITDRGRPIALLSPMPEGGPYQQMLASGGIERATLDFDDLPEPVRLKTGIELPS
VTLARLREHER

Download         Length: 276 bp

>AT71855 NZ_CP019221:1073737-1074012 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTACGGTGGGCGTTCGTGAGCTGCGGCAGCGGGCCAGCGAACTCCTCCGTCGGGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGTGGTCGGCCTATCGCCCTTCTCTCGCCGATGCCGGAGGGCGGTCCGTACCAGCAGATGCTGGCAAGCG
GCGGGATCGAACGTGCGACGCTTGACTTTGATGACCTTCCCGAGCCGGTCCGACTCAAAACGGGTATCGAGCTGCCGTCC
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X1XV84

Antitoxin

Source ID Structure
AlphaFold DB A0A1X1XUZ3

References