Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5117372..5117997 Replicon chromosome
Accession NZ_CP019219
Organism Klebsiella pneumoniae strain 1756

Toxin (Protein)


Gene name vapC Uniprot ID A0A483ITU7
Locus tag BWG70_RS26235 Protein ID WP_048334590.1
Coordinates 5117372..5117755 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag BWG70_RS26240 Protein ID WP_004150355.1
Coordinates 5117755..5117997 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWG70_RS26220 5114738..5115640 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
BWG70_RS26225 5115637..5116272 + 636 WP_076027700.1 formate dehydrogenase cytochrome b556 subunit -
BWG70_RS26230 5116269..5117198 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
BWG70_RS26235 5117372..5117755 - 384 WP_048334590.1 type II toxin-antitoxin system VapC family toxin Toxin
BWG70_RS26240 5117755..5117997 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
BWG70_RS26245 5118202..5119119 + 918 WP_004181612.1 alpha/beta hydrolase -
BWG70_RS26250 5119133..5120074 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
BWG70_RS26255 5120119..5120556 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
BWG70_RS26260 5120553..5121413 - 861 WP_023280688.1 virulence factor BrkB family protein -
BWG70_RS26265 5121407..5122006 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14381.60 Da        Isoelectric Point: 7.3178

>T71852 WP_048334590.1 NZ_CP019219:c5117755-5117372 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGITGVVTPYEIHPE

Download         Length: 384 bp

>T71852 NZ_CP019219:c5117755-5117372 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
AGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTACTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT71852 WP_004150355.1 NZ_CP019219:c5117997-5117755 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT71852 NZ_CP019219:c5117997-5117755 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483ITU7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References