Detailed information of TA system TA071831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 87628..88147
• Replicon: plasmid p41-1
• Accession: NZ_CP019209
• Organism: Enterococcus faecium strain 2014-VREF-41

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: BVA20_RS15840
• Protein ID: WP_000588503.1
• Coordinates: 87890..88147 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: BVA20_RS15835
• Protein ID: WP_000388479.1
• Coordinates: 87628..87897 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVA20_RS15780	82825..83724	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BVA20_RS15785	83727..84212	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
BVA20_RS15790	84353..84556	-	204	WP_002316074.1	putative holin-like toxin	-
BVA20_RS15795	84631..84891	+	261	WP_002295623.1	hypothetical protein	-
BVA20_RS15805	85328..85534	+	207	WP_002347218.1	hypothetical protein	-
BVA20_RS15810	85534..85785	+	252	WP_002296238.1	hypothetical protein	-
BVA20_RS15815	85800..86237	+	438	WP_002300851.1	hypothetical protein	-
BVA20_RS15820	86230..86937	+	708	WP_002303117.1	hypothetical protein	-
BVA20_RS15830	87318..87497	+	180	WP_001120991.1	hypothetical protein	-
BVA20_RS15835	87628..87897	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BVA20_RS15840	87890..88147	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
BVA20_RS17220	88606..88761	+	156	WP_002347142.1	hypothetical protein	-
BVA20_RS15845	88785..89609	+	825	WP_001809248.1	hypothetical protein	-
BVA20_RS15850	89774..90388	-	615	WP_001261742.1	recombinase family protein	-
BVA20_RS15855	90626..91048	+	423	WP_002303116.1	hypothetical protein	-
BVA20_RS15860	91058..91261	+	204	WP_002299573.1	hypothetical protein	-
BVA20_RS15865	91472..92056	+	585	WP_002299575.1	recombinase family protein	-
BVA20_RS15870	92245..92925	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..215998	215998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T71831 WP_000588503.1 NZ_CP019209:87890-88147 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T71831 NZ_CP019209:87890-88147 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT71831 WP_000388479.1 NZ_CP019209:87628-87897 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT71831 NZ_CP019209:87628-87897 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7