Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 172147..173057 Replicon plasmid pGN-2
Accession NZ_CP019161
Organism Klebsiella pneumoniae strain GN-2

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag BKY56_RS27790 Protein ID WP_004144067.1
Coordinates 172147..172617 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag BKY56_RS27795 Protein ID WP_004026357.1
Coordinates 172614..173057 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKY56_RS27750 167458..167724 - 267 WP_003026799.1 DUF1778 domain-containing protein -
BKY56_RS27755 167857..168285 - 429 WP_004901287.1 GFA family protein -
BKY56_RS27760 168486..168632 - 147 WP_159193595.1 IS3 family transposase -
BKY56_RS27765 168654..169774 + 1121 WP_101837575.1 IS3 family transposase -
BKY56_RS27770 169801..170616 - 816 WP_077268817.1 IS3 family transposase -
BKY56_RS27775 170571..171080 - 510 WP_004901294.1 IS3 family transposase -
BKY56_RS27785 171776..172036 + 261 WP_048291871.1 hypothetical protein -
BKY56_RS27790 172147..172617 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
BKY56_RS27795 172614..173057 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
BKY56_RS27800 173158..173664 - 507 Protein_158 DUF4113 domain-containing protein -
BKY56_RS27805 173765..173986 - 222 WP_072060588.1 DUF2188 domain-containing protein -
BKY56_RS27810 174410..175147 + 738 Protein_160 DUF1311 domain-containing protein -
BKY56_RS27815 175147..175488 + 342 WP_048291869.1 hypothetical protein -
BKY56_RS27825 175883..176928 + 1046 Protein_162 IS3 family transposase -
BKY56_RS29095 176983..177357 + 375 WP_173675457.1 hypothetical protein -
BKY56_RS29100 177287..177511 + 225 WP_127807699.1 DUF4113 domain-containing protein -
BKY56_RS27835 177590..177818 + 229 Protein_165 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..261986 261986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T71800 WP_004144067.1 NZ_CP019161:c172617-172147 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T71800 NZ_CP019161:c172617-172147 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT71800 WP_004026357.1 NZ_CP019161:c173057-172614 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT71800 NZ_CP019161:c173057-172614 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCAAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCTTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References