Detailed information of TA system TA071798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 129836..130361
• Replicon: plasmid pGN-2
• Accession: NZ_CP019161
• Organism: Klebsiella pneumoniae strain GN-2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: BKY56_RS27565
• Protein ID: WP_075647615.1
• Coordinates: 130056..130361 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: BKY56_RS27560
• Protein ID: WP_015632547.1
• Coordinates: 129836..130054 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKY56_RS27525	125262..125846	-	585	WP_023345591.1	TetR/AcrR family transcriptional regulator	-
BKY56_RS27530	125958..126353	-	396	WP_023345592.1	helix-turn-helix transcriptional regulator	-
BKY56_RS27535	126456..127301	+	846	WP_023345593.1	SDR family oxidoreductase	-
BKY56_RS27540	127414..127842	+	429	WP_023345594.1	nuclear transport factor 2 family protein	-
BKY56_RS27550	128184..129557	-	1374	WP_048265005.1	hypothetical protein	-
BKY56_RS27560	129836..130054	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
BKY56_RS27565	130056..130361	+	306	WP_075647615.1	type II toxin-antitoxin system toxin CcdB	Toxin
BKY56_RS27570	130548..131536	+	989	Protein_113	hypothetical protein	-
BKY56_RS27575	131735..132520	+	786	WP_048291886.1	site-specific integrase	-
BKY56_RS28605	132842..133147	-	306	WP_126034233.1	hypothetical protein	-
BKY56_RS27580	133296..133673	-	378	WP_158672279.1	DUF1173 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..261986	261986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 5.6844

>T71798 WP_075647615.1 NZ_CP019161:130056-130361 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHWENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T71798 NZ_CP019161:130056-130361 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAAGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACTGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT71798 WP_015632547.1 NZ_CP019161:129836-130054 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT71798 NZ_CP019161:129836-130054 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86