Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 108775..109579 Replicon plasmid pGN-2
Accession NZ_CP019161
Organism Klebsiella pneumoniae strain GN-2

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag BKY56_RS27440 Protein ID WP_072198182.1
Coordinates 109052..109579 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag BKY56_RS27435 Protein ID WP_048264996.1
Coordinates 108775..109044 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKY56_RS27420 105428..106675 - 1248 WP_048264960.1 nucleotidyltransferase -
BKY56_RS27425 106672..107847 - 1176 WP_077258344.1 SAVED domain-containing protein -
BKY56_RS27435 108775..109044 + 270 WP_048264996.1 DUF1778 domain-containing protein Antitoxin
BKY56_RS27440 109052..109579 + 528 WP_072198182.1 GNAT family N-acetyltransferase Toxin
BKY56_RS28600 109663..109971 + 309 WP_064167953.1 hypothetical protein -
BKY56_RS27450 110022..110719 + 698 Protein_92 IS1 family transposase -
BKY56_RS27455 111031..111933 - 903 WP_048265000.1 LysR family transcriptional regulator -
BKY56_RS27460 112031..112639 + 609 WP_023345578.1 short chain dehydrogenase -
BKY56_RS27465 112758..113939 - 1182 WP_023345579.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..261986 261986
- flank IS/Tn - - 110216..110719 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(54-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 20078.04 Da        Isoelectric Point: 5.7389

>T71796 WP_072198182.1 NZ_CP019161:109052-109579 [Klebsiella pneumoniae]
VMQELAIEMINDEFDYDIGEFDCGEETLNTFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Download         Length: 528 bp

>T71796 NZ_CP019161:109052-109579 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTAACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10148.65 Da        Isoelectric Point: 10.1733

>AT71796 WP_048264996.1 NZ_CP019161:108775-109044 [Klebsiella pneumoniae]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Download         Length: 270 bp

>AT71796 NZ_CP019161:108775-109044 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References