Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3861685..3862328 Replicon chromosome
Accession NZ_CP019160
Organism Klebsiella pneumoniae strain GN-2

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag BKY56_RS19305 Protein ID WP_032433387.1
Coordinates 3861685..3862101 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BKY56_RS19310 Protein ID WP_001261276.1
Coordinates 3862098..3862328 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKY56_RS19275 3856854..3857057 - 204 WP_032433376.1 hemolysin expression modulator Hha -
BKY56_RS19280 3857117..3857608 - 492 WP_040154632.1 hypothetical protein -
BKY56_RS19285 3857639..3858157 - 519 WP_048270189.1 hypothetical protein -
BKY56_RS19290 3858216..3858464 - 249 WP_032433382.1 hypothetical protein -
BKY56_RS19295 3858783..3860354 + 1572 WP_040025608.1 ATP-binding protein -
BKY56_RS19300 3860549..3861529 - 981 WP_032433385.1 hypothetical protein -
BKY56_RS19305 3861685..3862101 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
BKY56_RS19310 3862098..3862328 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BKY56_RS19320 3862633..3863448 + 816 WP_040025605.1 hypothetical protein -
BKY56_RS19330 3863976..3866216 - 2241 WP_045326788.1 NTPase -
BKY56_RS19335 3866219..3867306 - 1088 Protein_3660 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T71792 WP_032433387.1 NZ_CP019160:c3862101-3861685 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T71792 NZ_CP019160:c3862101-3861685 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT71792 WP_001261276.1 NZ_CP019160:c3862328-3862098 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT71792 NZ_CP019160:c3862328-3862098 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References