Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3844902..3845638 Replicon chromosome
Accession NZ_CP019160
Organism Klebsiella pneumoniae strain GN-2

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag BKY56_RS19205 Protein ID WP_032433360.1
Coordinates 3844902..3845384 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BKY56_RS19210 Protein ID WP_003026799.1
Coordinates 3845372..3845638 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BKY56_RS19185 3841451..3842191 + 741 WP_048978466.1 molecular chaperone -
BKY56_RS19190 3842195..3842773 + 579 WP_032432061.1 type 1 fimbrial protein -
BKY56_RS19195 3842785..3843729 + 945 WP_077256325.1 fimbrial protein -
BKY56_RS19200 3843942..3844541 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
BKY56_RS19205 3844902..3845384 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
BKY56_RS19210 3845372..3845638 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BKY56_RS19215 3845950..3846513 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
BKY56_RS19220 3846523..3847155 - 633 WP_040025617.1 3-hexulose-6-phosphate synthase -
BKY56_RS19225 3847173..3848177 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
BKY56_RS19230 3848161..3848823 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
BKY56_RS19235 3848852..3849991 - 1140 WP_040025615.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3836340..3860354 24014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T71791 WP_032433360.1 NZ_CP019160:c3845384-3844902 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T71791 NZ_CP019160:c3845384-3844902 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT71791 WP_003026799.1 NZ_CP019160:c3845638-3845372 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT71791 NZ_CP019160:c3845638-3845372 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References