71757

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2129979..2130508 Replicon chromosome
Accession NZ_CP019117
Organism Staphylococcus aureus strain SJTUF_J27

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag BRL61_RS11010 Protein ID WP_000621176.1
Coordinates 2129979..2130341 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag BRL61_RS11015 Protein ID WP_000948331.1
Coordinates 2130338..2130508 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BRL61_RS10985 2126957..2127727 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
BRL61_RS10990 2127702..2128181 - 480 WP_001190825.1 anti-sigma B factor RsbW -
BRL61_RS10995 2128183..2128509 - 327 WP_001052491.1 anti-sigma factor antagonist -
BRL61_RS11000 2128628..2129629 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
BRL61_RS11010 2129979..2130341 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BRL61_RS11015 2130338..2130508 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
BRL61_RS11020 2130593..2131741 - 1149 WP_001281140.1 alanine racemase -
BRL61_RS11025 2131807..2132166 - 360 WP_000581197.1 holo-ACP synthase -
BRL61_RS11030 2132170..2132661 - 492 WP_076050725.1 PH domain-containing protein -
BRL61_RS11035 2132654..2134231 - 1578 WP_053865710.1 PH domain-containing protein -
BRL61_RS11040 2134224..2134703 - 480 WP_001287082.1 hypothetical protein -
BRL61_RS11045 2134912..2135472 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T71757 WP_000621176.1 NZ_CP019117:c2130341-2129979 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T71757 NZ_CP019117:c2130341-2129979 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT71757 WP_000948331.1 NZ_CP019117:c2130508-2130338 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT71757 NZ_CP019117:c2130508-2130338 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References