Detailed information of TA system TA071715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 6101474..6102132
• Replicon: chromosome
• Accession: NZ_CP019082
• Organism: Paludisphaera borealis strain PX4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BSF38_RS23630
• Protein ID: WP_168189439.1
• Coordinates: 6101716..6102132 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BSF38_RS23625
• Protein ID: WP_076349562.1
• Coordinates: 6101474..6101713 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSF38_RS23610	6097008..6098540	+	1533	WP_076349559.1	hypothetical protein	-
BSF38_RS23615	6099089..6099856	-	768	WP_076349560.1	glucose 1-dehydrogenase	-
BSF38_RS23620	6100305..6101420	+	1116	WP_076349561.1	aminopeptidase	-
BSF38_RS23625	6101474..6101713	+	240	WP_076349562.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BSF38_RS23630	6101716..6102132	+	417	WP_168189439.1	type II toxin-antitoxin system VapC family toxin	Toxin
BSF38_RS23635	6102253..6102663	+	411	WP_076349564.1	hypothetical protein	-
BSF38_RS23640	6103013..6104140	+	1128	WP_083713346.1	DNA polymerase IV	-
BSF38_RS23645	6104320..6104616	-	297	WP_076349565.1	hypothetical protein	-
BSF38_RS23650	6104691..6106619	-	1929	WP_076349566.1	dihydroxy-acid dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-60)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14729.16 Da
• Isoelectric Point: 7.2794

>T71715 WP_168189439.1 NZ_CP019082:6101716-6102132 [Paludisphaera borealis]
VLLDTCVLAEIRLPEGDPLVKSAVREIPADDLYLSVLSVGEIAKGIALLAVGRKKKALSSWLVALETQFGDRILGVDVET
ARIWGELTARSQKTGVVIPASDGLLAATALRHGLHVMTRNTRHFEASGALLINPWPGA

Nucleotide

• Length: 417 bp

>T71715 NZ_CP019082:6101716-6102132 [Paludisphaera borealis]
GTGCTGCTTGACACCTGCGTCCTCGCGGAGATTCGGCTGCCCGAGGGCGATCCCTTGGTGAAGTCGGCGGTACGGGAGAT
TCCCGCGGACGACCTTTACCTTAGCGTGCTGTCCGTTGGCGAGATCGCCAAAGGCATCGCCCTGCTGGCCGTCGGCCGGA
AGAAGAAAGCCCTCTCCTCGTGGCTTGTGGCGCTCGAAACGCAGTTCGGCGACCGCATCCTCGGCGTCGACGTCGAGACG
GCCAGGATCTGGGGCGAATTGACGGCGCGATCGCAAAAGACCGGCGTCGTCATCCCCGCCTCCGACGGCCTCCTCGCCGC
AACCGCCCTCCGTCATGGGCTCCACGTCATGACCCGAAATACGCGTCACTTCGAGGCAAGCGGGGCGCTTCTCATCAACC
CTTGGCCGGGCGCGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8974.18 Da
• Isoelectric Point: 4.7719

>AT71715 WP_076349562.1 NZ_CP019082:6101474-6101713 [Paludisphaera borealis]
MTWKLAEAKNRLTEVVNLALTEGPQTITRRSDTVVVISAARYAELTGRKPDFKDFLFQGESFEGLDLTRDQSPMRDVEL

Nucleotide

• Length: 240 bp

>AT71715 NZ_CP019082:6101474-6101713 [Paludisphaera borealis]
ATGACCTGGAAACTTGCCGAAGCCAAAAACCGACTCACCGAAGTTGTGAACCTGGCGCTCACCGAGGGGCCGCAGACGAT
CACCAGGCGGAGTGATACGGTCGTCGTCATTTCGGCGGCGAGGTATGCCGAATTGACGGGTCGGAAGCCCGATTTCAAGG
ACTTTCTCTTCCAGGGGGAATCGTTCGAGGGGCTCGACCTGACGCGTGATCAGAGCCCGATGCGGGACGTCGAGTTGTGA