Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57666..58191 Replicon plasmid p1493-5
Accession NZ_CP019076
Organism Escherichia coli strain CRE1493

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BVL38_RS27095 Protein ID WP_001159871.1
Coordinates 57666..57971 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag BVL38_RS27100 Protein ID WP_000813630.1
Coordinates 57973..58191 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL38_RS27080 53628..54794 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BVL38_RS27085 55382..56137 - 756 WP_000852146.1 replication initiation protein RepE -
BVL38_RS27090 56859..57665 - 807 WP_000016970.1 site-specific integrase -
BVL38_RS27095 57666..57971 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BVL38_RS27100 57973..58191 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BVL38_RS27110 58751..58981 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BVL38_RS27115 58978..59394 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
BVL38_RS27120 59469..61034 + 1566 WP_001128474.1 AAA family ATPase -
BVL38_RS27125 61019..62041 + 1023 WP_000361402.1 helicase UvrD -
BVL38_RS27135 62295..62981 - 687 Protein_75 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) - 1..127772 127772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T71710 WP_001159871.1 NZ_CP019076:c57971-57666 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71710 NZ_CP019076:c57971-57666 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT71710 WP_000813630.1 NZ_CP019076:c58191-57973 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71710 NZ_CP019076:c58191-57973 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References