Detailed information of TA system TA071705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 69118..69719
• Replicon: plasmid p1493-4
• Accession: NZ_CP019075
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: BVL38_RS26585
• Protein ID: WP_001216045.1
• Coordinates: 69339..69719 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: BVL38_RS26580
• Protein ID: WP_001190712.1
• Coordinates: 69118..69339 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS26530	64304..64567	+	264	WP_000224220.1	hypothetical protein	-
BVL38_RS26535	64578..65177	+	600	WP_053903357.1	DUF551 domain-containing protein	-
BVL38_RS26540	65260..65493	+	234	WP_000516537.1	hypothetical protein	-
BVL38_RS26545	65672..65965	+	294	WP_001677496.1	hypothetical protein	-
BVL38_RS26550	65972..66346	+	375	WP_023154376.1	hypothetical protein	-
BVL38_RS26555	66328..67260	+	933	WP_077780118.1	hypothetical protein	-
BVL38_RS26560	67257..67619	+	363	WP_001261544.1	hypothetical protein	-
BVL38_RS28105	67694..67831	-	138	WP_000123562.1	hypothetical protein	-
BVL38_RS26570	68281..68532	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
BVL38_RS26575	68656..69045	+	390	WP_000506726.1	DNA repair protein	-
BVL38_RS26580	69118..69339	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BVL38_RS26585	69339..69719	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BVL38_RS26590	69724..69903	+	180	WP_000113019.1	hypothetical protein	-
BVL38_RS26595	69931..70974	+	1044	WP_000648823.1	DUF968 domain-containing protein	-
BVL38_RS26600	71063..71515	+	453	WP_001369802.1	hypothetical protein	-
BVL38_RS26605	71602..72795	+	1194	WP_000219605.1	terminase	-
BVL38_RS26610	72795..74279	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96986	96986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T71705 WP_001216045.1 NZ_CP019075:69339-69719 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T71705 NZ_CP019075:69339-69719 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT71705 WP_001190712.1 NZ_CP019075:69118-69339 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT71705 NZ_CP019075:69118-69339 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6