Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 28734..29326 Replicon plasmid p1493-1
Accession NZ_CP019072
Organism Escherichia coli strain CRE1493

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag BVL38_RS25235 Protein ID WP_000520549.1
Coordinates 29078..29326 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag BVL38_RS25230 Protein ID WP_000681613.1
Coordinates 28734..29081 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL38_RS25195 24739..25011 - 273 WP_001328551.1 TrbC/VirB2 family protein -
BVL38_RS25200 25079..25699 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
BVL38_RS25205 26055..26270 - 216 WP_000760375.1 EexN family lipoprotein -
BVL38_RS25210 26267..26560 - 294 WP_000134733.1 LuxR family transcriptional regulator -
BVL38_RS25215 26637..27866 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
BVL38_RS25220 27870..28373 - 504 WP_000781813.1 hypothetical protein -
BVL38_RS25230 28734..29081 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BVL38_RS25235 29078..29326 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
BVL38_RS25240 29631..29981 - 351 WP_000854259.1 hypothetical protein -
BVL38_RS25245 30072..30371 - 300 WP_000650309.1 hypothetical protein -
BVL38_RS25255 30825..31052 - 228 WP_000801440.1 hypothetical protein -
BVL38_RS25260 31039..31290 - 252 WP_001230707.1 hypothetical protein -
BVL38_RS25265 31287..31830 - 544 Protein_39 hypothetical protein -
BVL38_RS27835 31935..32450 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33858 33858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T71703 WP_000520549.1 NZ_CP019072:c29326-29078 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T71703 NZ_CP019072:c29326-29078 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT71703 WP_000681613.1 NZ_CP019072:c29081-28734 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT71703 NZ_CP019072:c29081-28734 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References