Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4356041..4356538 Replicon chromosome
Accession NZ_CP019071
Organism Escherichia coli strain CRE1493

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BVL38_RS22440 Protein ID WP_001173343.1
Coordinates 4356041..4356214 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BVL38_RS22445 Protein ID WP_000398619.1
Coordinates 4356242..4356538 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL38_RS22430 4352711..4354168 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
BVL38_RS22435 4354405..4355922 + 1518 WP_001295074.1 lysine--tRNA ligase -
BVL38_RS22440 4356041..4356214 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BVL38_RS22445 4356242..4356538 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BVL38_RS22455 4356765..4357037 - 273 WP_000405651.1 N-acetyltransferase -
BVL38_RS22460 4357049..4357279 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
BVL38_RS22465 4357460..4359091 + 1632 WP_001216477.1 sensor histidine kinase -
BVL38_RS22470 4359088..4359807 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T71702 WP_001173343.1 NZ_CP019071:c4356214-4356041 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T71702 NZ_CP019071:c4356214-4356041 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT71702 WP_000398619.1 NZ_CP019071:c4356538-4356242 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT71702 NZ_CP019071:c4356538-4356242 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References