Detailed information of TA system TA071697

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3702528..3703222
• Replicon: chromosome
• Accession: NZ_CP019071
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: BVL38_RS18975
• Protein ID: WP_001094398.1
• Coordinates: 3702528..3702896 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: BVL38_RS18980
• Protein ID: WP_077249034.1
• Coordinates: 3702917..3703222 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS18955	3697701..3698657	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
BVL38_RS18960	3698654..3700852	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
BVL38_RS18965	3700862..3701818	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
BVL38_RS18970	3701869..3702207	+	339	Protein_3461	LysR family transcriptional regulator	-
BVL38_RS18975	3702528..3702896	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
BVL38_RS18980	3702917..3703222	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BVL38_RS18985	3703326..3703970	-	645	WP_000086755.1	hypothetical protein	-
BVL38_RS18990	3703989..3704210	-	222	WP_000692345.1	DUF987 domain-containing protein	-
BVL38_RS18995	3704279..3704755	-	477	WP_001424026.1	RadC family protein	-
BVL38_RS19000	3704771..3705244	-	474	WP_000855059.1	antirestriction protein	-
BVL38_RS19010	3705586..3706407	-	822	WP_001234565.1	DUF945 domain-containing protein	-
BVL38_RS27775	3706508..3706716	-	209	Protein_3469	DUF905 domain-containing protein	-
BVL38_RS19020	3706817..3707272	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T71697 WP_001094398.1 NZ_CP019071:c3702896-3702528 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T71697 NZ_CP019071:c3702896-3702528 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT71697 WP_077249034.1 NZ_CP019071:c3703222-3702917 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT71697 NZ_CP019071:c3703222-3702917 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4