Detailed information of TA system TA071685

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1866618..1867453
• Replicon: chromosome
• Accession: NZ_CP019071
• Organism: Escherichia coli strain CRE1493

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BVL38_RS09465
• Protein ID: WP_042046912.1
• Coordinates: 1866618..1866995 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BVL38_RS09470
• Protein ID: WP_135145692.1
• Coordinates: 1867085..1867453 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL38_RS09430	1862138..1862611	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
BVL38_RS09435	1862809..1863867	+	1059	WP_001200895.1	FUSC family protein	-
BVL38_RS09440	1864039..1864368	+	330	WP_000450409.1	DUF496 family protein	-
BVL38_RS09445	1864469..1864651	-	183	WP_024198358.1	ethanolamine utilization protein	-
BVL38_RS27955	1865105..1865452	-	348	WP_001333417.1	IS110 family transposase	-
BVL38_RS27960	1866292..1866405	-	114	WP_032140621.1	DUF957 domain-containing protein	-
BVL38_RS09460	1866418..1866621	-	204	WP_000761705.1	hypothetical protein	-
BVL38_RS09465	1866618..1866995	-	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
BVL38_RS09470	1867085..1867453	-	369	WP_135145692.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BVL38_RS09475	1867616..1867837	-	222	WP_000692345.1	DUF987 domain-containing protein	-
BVL38_RS09480	1867900..1868376	-	477	WP_001186774.1	RadC family protein	-
BVL38_RS09485	1868392..1868865	-	474	WP_000855059.1	antirestriction protein	-
BVL38_RS09495	1869207..1870025	-	819	WP_001234729.1	DUF945 domain-containing protein	-
BVL38_RS09505	1870180..1870338	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T71685 WP_042046912.1 NZ_CP019071:c1866995-1866618 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T71685 NZ_CP019071:c1866995-1866618 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13545.30 Da
• Isoelectric Point: 6.4764

>AT71685 WP_135145692.1 NZ_CP019071:c1867453-1867085 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYAYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71685 NZ_CP019071:c1867453-1867085 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA