Detailed information of TA system TA071677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3617081..3617721
• Replicon: chromosome
• Accession: NZ_CP019066
• Organism: Tsukamurella tyrosinosolvens strain MH1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ASU32_RS17620
• Protein ID: WP_068523248.1
• Coordinates: 3617347..3617721 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ASU32_RS17615
• Protein ID: WP_102102512.1
• Coordinates: 3617081..3617335 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASU32_RS17600	3612545..3613216	+	672	WP_102102511.1	hypothetical protein	-
ASU32_RS17605	3613245..3615998	-	2754	WP_082809245.1	preprotein translocase subunit SecA	-
ASU32_RS17610	3616078..3616803	-	726	WP_068522205.1	ribosome-associated translation inhibitor RaiA	-
ASU32_RS17615	3617081..3617335	+	255	WP_102102512.1	antitoxin	Antitoxin
ASU32_RS17620	3617347..3617721	+	375	WP_068523248.1	type II toxin-antitoxin system VapC family toxin	Toxin
ASU32_RS17625	3617823..3618473	-	651	WP_068626524.1	ComF family protein	-
ASU32_RS17630	3618549..3620345	-	1797	WP_068522210.1	MtrAB system accessory protein LpqB	-
ASU32_RS17635	3620338..3621915	-	1578	WP_082791559.1	HAMP domain-containing histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13635.45 Da
• Isoelectric Point: 6.4771

>T71677 WP_068523248.1 NZ_CP019066:3617347-3617721 [Tsukamurella tyrosinosolvens]
ILLDTSIFIDTLRGRREARDRLSDARREGRRLVASVLTRTEVLGGMRASEKSSTAALLAVFEWIDVSEEIADAAGSLARR
YRASHSGIDIADYVIAATAAVAGADLWTRNVKHFPMFDGLEPPY

Nucleotide

• Length: 375 bp

>T71677 NZ_CP019066:3617347-3617721 [Tsukamurella tyrosinosolvens]
ATCCTCCTCGACACGTCGATCTTCATCGATACCCTCCGCGGGCGGAGGGAGGCTCGGGACCGCCTGTCGGATGCGCGCCG
GGAGGGACGGCGCCTCGTCGCGTCGGTCCTCACCCGCACCGAGGTCCTCGGCGGTATGCGCGCCTCCGAGAAGTCGTCCA
CCGCCGCCCTCCTCGCGGTCTTCGAGTGGATCGACGTCTCAGAGGAGATCGCCGACGCCGCGGGGTCTCTGGCCCGCCGG
TACCGCGCGTCGCACTCCGGGATCGACATCGCTGACTACGTGATCGCGGCGACCGCCGCAGTCGCCGGTGCCGATCTCTG
GACCCGCAACGTCAAGCATTTCCCGATGTTCGACGGGCTCGAACCGCCGTACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9244.20 Da
• Isoelectric Point: 4.3055

>AT71677 WP_102102512.1 NZ_CP019066:3617081-3617335 [Tsukamurella tyrosinosolvens]
MSHRTQITLEDAQYVRLLAESRASGLGLAELIRRAVDLTYGGADADAFDAALDQSFGTWGSDGPDGEEFVEAIRPARIDR
FARW

Nucleotide

• Length: 255 bp

>AT71677 NZ_CP019066:3617081-3617335 [Tsukamurella tyrosinosolvens]
ATGAGCCATCGGACGCAGATCACGCTCGAGGATGCGCAGTACGTTCGCCTCCTCGCGGAGTCCCGCGCGTCCGGGCTCGG
GCTGGCCGAACTGATCCGCCGCGCCGTCGATCTGACCTACGGCGGAGCCGACGCGGACGCGTTCGACGCGGCACTCGACC
AGAGCTTCGGGACATGGGGATCCGACGGGCCCGACGGCGAAGAGTTCGTAGAGGCGATCCGACCTGCACGGATTGATCGG
TTCGCCCGGTGGTAA