Detailed information of TA system TA071653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 178022..178557
• Replicon: plasmid p1540-4
• Accession: NZ_CP019055
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: BVL39_RS28740
• Protein ID: WP_000222760.1
• Coordinates: 178270..178557 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: BVL39_RS28735
• Protein ID: WP_001132900.1
• Coordinates: 178022..178273 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS28690	173149..173709	+	561	WP_000139315.1	fertility inhibition protein FinO	-
BVL39_RS28695	173845..174057	+	213	WP_001299730.1	ANR family transcriptional regulator	-
BVL39_RS29380	174303..174377	+	75	Protein_179	endonuclease	-
-	174633..174694	-	62	NuclAT_1	-	-
-	174633..174694	-	62	NuclAT_1	-	-
-	174633..174694	-	62	NuclAT_1	-	-
-	174633..174694	-	62	NuclAT_1	-	-
BVL39_RS28705	174681..174887	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
BVL39_RS28710	175171..175428	+	258	WP_000083845.1	replication regulatory protein RepA	-
BVL39_RS28720	175660..175734	+	75	WP_001442103.1	RepA leader peptide Tap	-
BVL39_RS28725	175727..176209	+	483	WP_001273588.1	hypothetical protein	-
BVL39_RS28730	176202..177059	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
BVL39_RS28735	178022..178273	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BVL39_RS28740	178270..178557	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BVL39_RS28750	178997..179344	-	348	Protein_187	IS1-like element IS1A family transposase	-
BVL39_RS28760	179433..180455	+	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
BVL39_RS28765	180452..181234	+	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
BVL39_RS28770	181289..181653	-	365	Protein_190	IS1 family transposase	-
BVL39_RS29115	182318..182638	+	321	WP_001334006.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..184166	184166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T71653 WP_000222760.1 NZ_CP019055:178270-178557 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T71653 NZ_CP019055:178270-178557 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT71653 WP_001132900.1 NZ_CP019055:178022-178273 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT71653 NZ_CP019055:178022-178273 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2