Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 117211..117736 Replicon plasmid p1540-4
Accession NZ_CP019055
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BVL39_RS28355 Protein ID WP_001159871.1
Coordinates 117431..117736 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag BVL39_RS28350 Protein ID WP_000813630.1
Coordinates 117211..117429 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS28315 114184..114600 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
BVL39_RS28320 114597..114827 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BVL39_RS28330 115246..115935 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
BVL39_RS28335 115966..116655 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
BVL39_RS28350 117211..117429 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BVL39_RS28355 117431..117736 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BVL39_RS28360 117737..118483 + 747 WP_072748653.1 site-specific integrase -
BVL39_RS28365 119183..119332 + 150 Protein_117 RepB family plasmid replication initiator protein -
BVL39_RS28375 120738..121184 + 447 WP_000616196.1 hypothetical protein -
BVL39_RS28380 121280..121537 - 258 WP_015387373.1 hypothetical protein -
BVL39_RS28385 122266..122478 + 213 WP_001523378.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..184166 184166


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T71643 WP_001159871.1 NZ_CP019055:117431-117736 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71643 NZ_CP019055:117431-117736 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT71643 WP_000813630.1 NZ_CP019055:117211-117429 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71643 NZ_CP019055:117211-117429 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References