Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 114184..114827 Replicon plasmid p1540-4
Accession NZ_CP019055
Organism Escherichia coli strain CRE1540

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag BVL39_RS28315 Protein ID WP_001044768.1
Coordinates 114184..114600 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag BVL39_RS28320 Protein ID WP_001261287.1
Coordinates 114597..114827 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVL39_RS28295 109214..110779 - 1566 WP_001128474.1 AAA family ATPase -
BVL39_RS28300 110854..111270 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
BVL39_RS28305 111267..111497 - 231 WP_072647566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BVL39_RS28310 111884..114022 + 2139 WP_001513523.1 AAA family ATPase -
BVL39_RS28315 114184..114600 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
BVL39_RS28320 114597..114827 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BVL39_RS28330 115246..115935 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
BVL39_RS28335 115966..116655 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
BVL39_RS28350 117211..117429 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
BVL39_RS28355 117431..117736 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
BVL39_RS28360 117737..118483 + 747 WP_072748653.1 site-specific integrase -
BVL39_RS28365 119183..119332 + 150 Protein_117 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..184166 184166


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T71642 WP_001044768.1 NZ_CP019055:c114600-114184 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T71642 NZ_CP019055:c114600-114184 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGATGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT71642 WP_001261287.1 NZ_CP019055:c114827-114597 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT71642 NZ_CP019055:c114827-114597 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGCTCATTCCTGGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References