Detailed information of TA system TA071641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 110854..111497
• Replicon: plasmid p1540-4
• Accession: NZ_CP019055
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: V0UN72
• Locus tag: BVL39_RS28300
• Protein ID: WP_001034044.1
• Coordinates: 110854..111270 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: BVL39_RS28305
• Protein ID: WP_072647566.1
• Coordinates: 111267..111497 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS28280	107256..107953	+	698	Protein_104	IS1 family transposase	-
BVL39_RS28290	108207..109229	-	1023	WP_000361402.1	helicase UvrD	-
BVL39_RS28295	109214..110779	-	1566	WP_001128474.1	AAA family ATPase	-
BVL39_RS28300	110854..111270	-	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	Toxin
BVL39_RS28305	111267..111497	-	231	WP_072647566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BVL39_RS28310	111884..114022	+	2139	WP_001513523.1	AAA family ATPase	-
BVL39_RS28315	114184..114600	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	-
BVL39_RS28320	114597..114827	-	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BVL39_RS28330	115246..115935	+	690	WP_001513524.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..184166	184166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.66 Da
• Isoelectric Point: 6.8536

>T71641 WP_001034044.1 NZ_CP019055:c111270-110854 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71641 NZ_CP019055:c111270-110854 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8760.95 Da
• Isoelectric Point: 4.6437

>AT71641 WP_072647566.1 NZ_CP019055:c111497-111267 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKAEPDFMTERKDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT71641 NZ_CP019055:c111497-111267 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGAGCCGGACTTTATGACGGAGCGCAAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CHW1