Detailed information of TA system TA071636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 12833..13334
• Replicon: plasmid p1540-1
• Accession: NZ_CP019052
• Organism: Escherichia coli strain CRE1540

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: BVL39_RS25640
• Protein ID: WP_000702247.1
• Coordinates: 12833..13093 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: BVL39_RS25645
• Protein ID: WP_001132407.1
• Coordinates: 13083..13334 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVL39_RS25605	8955..10217	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
BVL39_RS25615	10976..11305	+	330	WP_000855120.1	hypothetical protein	-
BVL39_RS25625	11886..12209	+	324	WP_000427403.1	hypothetical protein	-
BVL39_RS25630	12251..12460	+	210	WP_000866036.1	hypothetical protein	-
BVL39_RS25635	12557..12781	+	225	WP_000814381.1	hypothetical protein	-
BVL39_RS25640	12833..13093	+	261	WP_000702247.1	hypothetical protein	Toxin
BVL39_RS25645	13083..13334	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
BVL39_RS25660	13884..14162	+	279	WP_000545938.1	hypothetical protein	-
BVL39_RS25665	14582..14848	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
BVL39_RS25670	14955..15137	-	183	WP_001481829.1	hypothetical protein	-
BVL39_RS25675	15695..15877	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
BVL39_RS25680	15902..16339	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
BVL39_RS25685	16470..16796	-	327	WP_063078684.1	hypothetical protein	-
BVL39_RS25690	16816..17523	-	708	WP_021503228.1	hypothetical protein	-
BVL39_RS25695	17525..18277	-	753	WP_063078685.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-55 / mcr-1.1	-	1..65533	65533
	-	flank	IS/Tn	blaCTX-M-55	-	7827..10217	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T71636 WP_000702247.1 NZ_CP019052:12833-13093 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T71636 NZ_CP019052:12833-13093 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT71636 WP_001132407.1 NZ_CP019052:13083-13334 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT71636 NZ_CP019052:13083-13334 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0